m_u

Stability/Folding

Slope of the Denaturant Unfolding Curve Cooperativity Value (m)

kcal/molĀ·M

Circular Dichroism (CD)

295 K

6.0

In all experiments, protein solutions were made in 50 mM sodium phosphate, pH 6.0, and experiments were carried out at 295 K. Equilibrium circular dichroism to determine protein stability and stopped-flow kinetic experiments were carried out as described