Fitness metric
Binding
Score
unitless
Illumina Sequencing
None
To normalize the data across the multiple tiles, we define the fitness metric for variant i (ζ i) as the binary logarithm of the mean fluorescence of variant i ( F i ) to the mean fluorescence of the wild-type sequence (F wt) (Kowalski et al., PLoS One 2015;10).
ζ i = log2 (F i / F wt)
Statistical analysis, from cited ref 14 (Kowalski et al, J Biol Chem 2015), indicates errors reported for all experiments are STD.
N/A = not applicable
NS = not significant? (counts unselected ≤ 10?)
NaN = ?