Resistance

Growth/Fitness

Resistance (susceptible, intermediate, resistant)

Resistance categories (SIR)

Etest

Mueller–Hinton agar

37 C

7.0

NA

NA

None

Genes encoding the NDM variants NDM-1, -2, -4, -5, -6 and -7 were amplified using DNA templates prepared from NDM-producing clinical isolates or E. coli transconjugants.(22–24) Both full coding sequences and the NDM gene with the native ISAb125 promoter (22) were amplified and cloned in the pCR-Blunt II TOPO vector (Invitrogen, Paisley, UK) and transformed in E. coli TOP10. The susceptibility of the transformants containing pCR2.1 NDM and pCR2.1 NDM P+ plasmids to ampicillin, cefalotin, cefoxitin, ceftazidime, ertapenem, imipenem, meropenem and doripenem was determined using the Etest method (bioMérieux, Basingstoke, UK) on Mueller–Hinton agar.

NDM-3 (D95N) and NDM-8 (M154L+D130G) variants were transformed in E. coli DH5α instead of E. coli TOP10

ISAb125 promoter genes included in this upload.

Reference:
22 Hornsey M Phee L Wareham DW A novel variant, NDM-5, of the New Delhi metallo-β-lactamase in a multidrug-resistant Escherichia coli ST648 isolate recovered from a patient in the United Kingdom Antimicrob Agents Chemother 2011 55 5952 4

23 Göttig S Hamprecht AG Christ S et al. Detection of NDM-7 in Germany, a new variant of the New Delhi metallo-β-lactamase with increased carbapenemase activity J Antimicrob Chemother 2013 68 1737 40

24 Tada T Miyoshi-Akiyama T Dahal RK et al. NDM-8 metallo-β-lactamase in a multidrug-resistant Escherichia coli strain isolated in Nepal Antimicrob Agents Chemother 2013 57 2394 6