Name:

Resistance

Category:

Growth/Fitness

Property:

Resistance (susceptible, intermediate, resistant)

Units:

Resistance categories (SIR)

Measurement Technique:

Broth Dilution/Turbidity

Buffers:

Mueller Hinton II broth

Temperature:

37°C

pH:

7.0

Protein Concentration:

N/A

Wavelength:

N/A

Ionic Strength:

None

Details:

Susceptibility profiles were determined six times in a broth microdilution scheme, following CLSI guidelines (37), using E. coli DH10B cells. The median MIC values are reported.

Reference:
37. Clinical and Laboratory Standards Institute. 2012. Methods for dilution
antimicrobial susceptibility tests for bacteria that grow aerobically; approved standard, ninth edition. M07-A9. Clinical and Laboratory Standards Institute, Wayne, PA.

SIR (susceptible, intermediate, or resistant) were determined based on CLSI breakpoints.