Solubility

Solubility/Aggregation

Soluble Fraction

unitless

SDS-PAGE

MOPS, 100 uM ZnSO4

22°C

7.0

variable (5-15 mg/L)

None

None

E. coli OverExpress C43 (DE3) cells (Lucigen, Middleton, WI) were transformed with the pET26b plasmids carrying blaIMP-1-V67A, blaIMP-1-V67I, and blaIMP-10 via electroporation. The enzymes were overexpressed for purification. The amount of soluble protein in the supernatant and insoluble protein in the pellet after cell lysis by sonication and centrifugation were compared to estimate the fraction of soluble protein.