Tm2

Stability/Folding

Melting Temperature (Tm)

°C (Celsius)

Thermal Denaturation , Differential Scanning Fluorimetry (DSF)

None

20-95 °C with 1 °C/min increments

None

None

330, 350 nm

None

Thermal denaturation was measured in high sensitivity capillaries using a Prometheus NT.48 instrument (NanoTemper Technologies). Fluorescence at 350 and 330 nm was collected and the unfolding temperatures (Tm) were determined from the first derivative of the fluorescence ratio (F350/F330). Scattering onset was measured using integrated back reflection optics of the instrument. Data processing was performed using PR control (version: 1.11; NanoTemper Technologies).