ΔSvh
Stability/Folding
van't Hoff Entropy (ΔS)
kcal/mol·K
Fluorescence Intensity
None
None
None
None
None
None
Fluorescence intensity ratio at 350 nm: 330 nm vs. temperature was fitted to a two-state transition model:
IT = {IN+aT+(ID+bT)exp[(ΔHvh/R)(1/Tm−1/T)]} / {1+exp[(ΔHvh/R)(1/Tm−1/T)]}
where IT is the observed signal; IN and ID, the native and denatured baseline intercepts; a and b, the native and denatured baseline slopes; T, the temperature; ∆Hvh, the van’t Hoff enthalpy; R, the gas constant (1.987 cal⋅mol−1⋅K−1); and Tm, the thermal transition midpoint. The van’t Hoff entropy was calculated using:
ΔSvh=ΔHvh/Tm