T50^15

Stability/Folding

Temperature of Half Activity (T50)

°C (Celsius)

Chromatography (HPLC, TLC)

None

20-65 °C

None

None

None

None

T50^15, the temperature required to reduce the initial enzyme activity by 50% within 15 min, was measured by placing 100 µL of enzymes at various temperatures, from 22 to 65 °C, for 15 min. T50^15 was determined from the inflection point of residual activities vs. temperature using a sigmoidal Boltzmann fit in OriginPro 9.0.