kcat

Activity

Catalytic Rate Constant (kcat)

1/s

Chromatography (HPLC, TLC)

50 mM Tris-HCl

60 °C

7.0

None

None

None

Reactions were initiated by addition of pre-warmed 50 μL of 150 mM Li-hydroxypyruvate (HPA), 150 mM glycolaldehyde (GA) in 50 mM Tris-HCl, pH 7.0. Aliquots of 10 μL were quenched at various times over 120 min with 190 μL of 0.1% (v/v) trifluoroacetic acid (TFA). Triplicate reactions were analysed by HPLC.