Name:

Tm pH5.0

Category:

Stability/Folding

Property:

Melting Temperature (Tm)

Units:

°C (Celsius)

Measurement Technique:

Circular Dichroism (CD)

Buffers:

50 mM sodium phosphate

Temperature:

pH:

5.0

Protein Concentration:

Wavelength:

222 nm

Ionic Strength:

Details:

CD data were collected on an Aviv 62DS spectrometer equipped with a thermoelectric unit. Protein samples were studied in 50 mM sodium phosphate buffer at pH 5.0. Wavelength scans were performed at 1°C. Thermal denaturations were monitored by CD at 222 nm and fitted to a two-state model as described (Minor & Kim, 1994).