Tm pH5.0


Melting Temperature (Tm)

°C (Celsius)

Circular Dichroism (CD)

50 mM sodium phosphate


222 nm

CD data were collected on an Aviv 62DS spectrometer equipped with a thermoelectric unit. Protein samples were studied in 50 mM sodium phosphate buffer at pH 5.0. Wavelength scans were performed at 1°C. Thermal denaturations were monitored by CD at 222 nm and fitted to a two-state model as described (Minor & Kim, 1994).