Name:

kcat

Category:

Activity

Property:

Catalytic Rate Constant (kcat)

Units:

1/s

Measurement Technique:

Spectrophotometric/Colorimetric Assay

Buffers:

25 mM sodium phosphate

Temperature:

25 °C

pH:

7.0

Protein Concentration:

None

Wavelength:

None

Ionic Strength:

None

Details:

The data were collected with a Perkin-Elmer Lambda 25 spectrophotometer (Perkin-Elmer Italia, Monza, Italy). Steady-state kinetic analyses were performed under initial-rate conditions using the Hanes plot linearization method. For Km values of ≤10 μM, the Km values were determined as Ki values, using nitrocefin as the reporter substrate. Each kinetic value is the mean of five different measurements; errors were <5%.