Binding: Kd

Binding

Dissociation Constant (Kd)

nM (nanoMolar)

Surface Plasmon Resonance (SPR)

10 mM sodium acetate, 150 mM NaCl, 0.05% surfactant Tween 20, pH 5.0

None

7.4

1 μM

None

None

Human IgG dissolved in HBS-T buffer (10 mM HEPES, 150 mM NaCl, 0.05% surfactant Tween 20, pH 7.4) was injected, and then dissociated by acidic buffer (10 mM sodium acetate, 150 mM NaCl, 0.05% surfactant Tween 20, pH 5.0).