Binding: koff

Binding

Rate Constant of Dissociation (koff)

1/s

Surface Plasmon Resonance (SPR)

10 mM sodium acetate, 150 mM NaCl, 0.05% surfactant Tween 20, pH 5.0

None

5.0

1 μM

None

None

Human IgG dissolved in HBS-T buffer (10 mM HEPES, 150 mM NaCl, 0.05% surfactant Tween 20, pH 7.4) was injected, and then dissociated by acidic buffer (10 mM sodium acetate, 150 mM NaCl, 0.05% surfactant Tween 20, pH 5.0).