Elution peak: Affinity chromatography

Expression

Peak pH of Elution Curve

unitless

Absorbance , Chromatography (HPLC, TLC)

TST buffer (50 mM Tris–HCl pH 7.6, 150 mM NaCl, 0.05% Tween20)

None

None

None

280 nm

None

The captured IgG was eluted with a decreasing pH gradient by using 50 mM trisodium citrate (pH 7.0) and 0.5 M acetate (pH 2.5).