Elution peak: Affinity chromatography
Peak pH of Elution Curve
Absorbance , Chromatography (HPLC, TLC)
TST buffer (50 mM Tris–HCl pH 7.6, 150 mM NaCl, 0.05% Tween20)
The captured IgG was eluted with a decreasing pH gradient by using 50 mM trisodium citrate (pH 7.0) and 0.5 M acetate (pH 2.5).