Temperature of Half Activity (T50)
Berthelot Reaction Method
200 mM phosphate–citrate buffer, 10 mM L-MSG, 0.1 mM PLP
T15_50 stands for temperature that the enzyme reduced to 50% of its initial enzyme activity after the heat treatment for 15 min. It was investigated by measuring the residual activity after incubating the enzyme solution at different temperatures for specific time. For T15_50 determination, the enzyme solutions were incubation at 20–70 °C for 15 min and then cooled on the ice for 5 min immediately.