t1/2 at 45 °C

Stability/Folding

Half-life (t1/2)

min

Berthelot Reaction Method

200 mM phosphate–citrate buffer

45 °C

3.8

1 mg/mL

None

None

The half-life time (t1/2) means the time when the enzyme reduced to 50% of its initial enzyme activity at specific temperature. Half-life was estimated by the plots of natural logs of residual activities versus the thermal-incubation time.