Recombinant protein content

Expression

Recombinant Protein Content

mg

ELISA , DAC-ELISA

Phosphate Buffered Saline, Tween20

None

None

None

None

None

Aliquots of protein samples were coated in wells of microtiter plate followed by blocking and incubation with 1:5000 dilution of rabbit anti-human α1-PI antibody, washing with PBST and incubation with 1:8000 dilution of goat anti-rabbit IgG-alkaline phosphatase conjugated antibody followed by colour development with p-nitrophenyl phosphate substrate solution. Expression levels of recombinant α1-PI were quantified on a linear standard curve plotted with pure human α1-PI protein.