Pro.v4-Tanezumab/mAbs Binding

Binding

Normalized binding score

Unitless

comprehensive mutagenesis , fluorescence-activated cell sorting , Yeast Surface Display , Deep Mutational Scanning

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The relative binding score for each mutant is defined as :

Relative binding score (i) = log2 ( mean fluorescence of variant i / relative mean fluorescence of the wild-type construct)

Detail protocols can be found via: Medina-Cucurella A.V., Whitehead T.A. (2018) Characterizing Protein-Protein Interactions Using Deep Sequencing Coupled to Yeast Surface Display. In: Marsh J. (eds) Protein Complex Assembly. Methods in Molecular Biology, vol 1764. Humana Press, New York, NY