dG_H2O

Stability/Folding

Gibbs Free Energy of Folding/Unfolding (ΔG)

kcal/mol

Fluorescence Spectroscopy , Urea Denaturation

Tris-HCl: 50 mM

25.0 C

7.5

2 micro M

None

:

Additives DTT (1 mM),