dG_H2O

Stability/Folding

Gibbs Free Energy of Folding/Unfolding (ΔG)

kcal/mol

Fluorescence Spectroscopy , Urea Denaturation

Tris-HCl: 50 mM

10.0 C

7.5

4 microM

None

:

Additives EDTA (1 mM),