Ka/KaAAA-44Thr -- Relative binding constant to human Fc AAA-44Thr

Stability/Folding

Equilibrium Constant (K)

unitless

Alkaline Phosphatase Assay

5 C

None

Fc binding was measured by adding variable amount of competitor protein to a fixed quantity of protein G-alkaline phosphatase in 96 well plates. The ratio of affinity constants for all the mutants was normalized to AAA-44Thr

as described previously in Minor DL Jr;Kim PS,Nature (1994) Measurement of the beta-sheet-forming propensities of amino acids. (study id =53)