Binding free energy

Binding

Gibbs Free Energy of Binding (ΔG)

kcal/mol

N_wt/N_Ala for antibody (3F6.B1.4B1) selection , N_wt/N_Ala for hGHbp selection

${ \Delta \Delta G_\mathrm{mut-wt}= \Delta \Delta G_\mathrm{hGHbp} - \Delta \Delta G_\mathrm{antibody} = RT\ln (N_\mathrm{WT}/N_\mathrm{Ala})_\mathrm{hGHbp} - RT\ln (N_\mathrm{WT}/N_\mathrm{Ala})_\mathrm{antibody} }$